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anti il 6 neutralizing antibody af506  (R&D Systems)


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    R&D Systems anti il 6 neutralizing antibody af506
    Anti Il 6 Neutralizing Antibody Af506, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rat+il+6+antibody/Rat+IL-6+Antibody/pm41290054-602-27-31
    Average 94 stars, based on 86 article reviews
    anti il 6 neutralizing antibody af506 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner
    Article Snippet: .. Special chemicals include ethyl pyruvate (EP, Sigma-Aldrich Co., Cat# E47808, Saint Louis, MO, USA), CLI-095 (Invivo Gen, Cat# tlrl-cli95, San Diego, CA, USA), C34 (TOCRIS, Cat# 5373, Bristol, UK), recombinant HMGB1 (rHMGB1, ProSpec, Cat# pro-581-b, Rehovot, Israel), BAY 11-7082 (Sigma-Aldrich Co., Cat# B5556), IL-6 (PeproTech Inc., Cat# 400-06-2UG, Rocky Hill, NJ, USA), and neutralizing anti-rat-IL-6 antibody (R&D SYSTEMS, Cat# AF506, Minneapolis, MN, USA). .. Primary antibodies include rabbit polyclonal anti-HMGB1 antibody for rat, mouse, and human (Abcam, Cat# ab18256, RRID:AB_444360, Cambridge, UK); rabbit polyclonal anti-AQP4 antibody for rat, mouse, human, and pig (Abcam, Cat# ab46182, RRID: AB_955676); mouse monoclonal anti-TLR4 antibody for rat, mouse, human, pig, baboon, bovine, and Chinese hamster (Novus, Cat# 76B357.1, RRID: AB_839000, Littleton, CO, USA); rabbit polyclonal anti-TLR4 antibody for rat, mouse, human, and rabbit (Boster, Cat# BA1717, RRID:AB_2716293); rabbit polyclonal anti-myeloid differentiation primary response gene 88 (MyD88) antibody for rat and human (Abcam, Cat# ab131071, RRID: AB_11156885); mouse monoclonal anti-IκBα antibody for rat, mouse, human, monkey, bovine, pig, and guinea pig (Cell Signaling Technology, Cat# 4814, RRID: AB_390781, Boston, MA, USA); mouse monoclonal anti-p-IκBα antibody for rat, mouse, human, and monkey (Cell Signaling Technology, Cat# 9246, RRID:AB_2267145); rabbit monoclonal anti-NF-κB antibody for rat, mouse, human, monkey, and bovine (Cell Signaling Technology, Cat# 4764, RRID:AB_823578); mouse monoclonal anti-GAPDH antibody for rat, mouse, and human (Beyotime, Cat# AF0006, RRID: AB_2715590, Shanghai, China); mouse monoclonal anti-Histone H3 antibody for rat, mouse, and human (Beyotime, Cat# AF0009, RRID: AB_2715593); and mouse monoclonal anti-S100β antibody for rat, mouse, human, rabbit, and pig (Boster, Cat# BM0120, RRID:AB_2716291).

    Article Title: Microglia/Macrophages and CD4 + CD25 + T Cells Enhance the Ability of Injury-Activated Lymphocytes to Reduce Traumatic Optic Neuropathy In Vitro
    Article Snippet: .. Monoclonal anti-rat TNF-α/TNFSF1A TNFR1/2 antibody and anti-rat IL-6 antibody, recombinant rat IL-6 and recombinant rat TNF-α protein (R&D Systems, Minneapolis, MN, USA) were added to the co-culture medium and cells were co-cultured for 7 days. ..

    Incubation:

    Article Title: Crosstalk between interleukin-6 and corticotropin-releasing factor modulate submucosal plexus activity and colonic secretion.
    Article Snippet: 0889-1591/$ see front matter 2013 Elsevier Inc. A http://dx.doi.org/10.1016/j.bbi.2013.01.078 Abbreviations: CRF, corticotropin releasing facto colo-rectal distension; GI, gastrointestinal; HPA, hyp IBS, irritable bowel syndrome; IL, interleukin; IL-6R short circuit current; JAK-STAT, janus tyrosine kinas activators of transcription; MAPK, mitogen activated separation; NS, non-separated; SD, standard deviatio mean; SMP, submucosal plexus; TER, trans-epithel necrosis factor.. ⇑ Corresponding author.. Address: Department o Gateway Building, University College Cork, Cork, Irela E-mail address: d.omalley@ucc.ie (D. O’Malley).

    Article Title: Colonic soluble mediators from the maternal separation model of irritable bowel syndrome activate submucosal neurons via an interleukin-6-dependent mechanism.
    Article Snippet: O’Malley D, Liston M, Hyland NP, Dinan TG, Cryan JF.. Colonic soluble mediators from the maternal separation model of irritable bowel syndrome activate submucosal neurons via an interleukin-6-dependent mechanism.. Am J Physiol Gastrointest Liver Physiol 300: G241–G252, 2011.

    Affinity Purification:

    Article Title: Crosstalk between interleukin-6 and corticotropin-releasing factor modulate submucosal plexus activity and colonic secretion.
    Article Snippet: 0889-1591/$ see front matter 2013 Elsevier Inc. A http://dx.doi.org/10.1016/j.bbi.2013.01.078 Abbreviations: CRF, corticotropin releasing facto colo-rectal distension; GI, gastrointestinal; HPA, hyp IBS, irritable bowel syndrome; IL, interleukin; IL-6R short circuit current; JAK-STAT, janus tyrosine kinas activators of transcription; MAPK, mitogen activated separation; NS, non-separated; SD, standard deviatio mean; SMP, submucosal plexus; TER, trans-epithel necrosis factor.. ⇑ Corresponding author.. Address: Department o Gateway Building, University College Cork, Cork, Irela E-mail address: d.omalley@ucc.ie (D. O’Malley).

    Article Title: Colonic soluble mediators from the maternal separation model of irritable bowel syndrome activate submucosal neurons via an interleukin-6-dependent mechanism.
    Article Snippet: O’Malley D, Liston M, Hyland NP, Dinan TG, Cryan JF.. Colonic soluble mediators from the maternal separation model of irritable bowel syndrome activate submucosal neurons via an interleukin-6-dependent mechanism.. Am J Physiol Gastrointest Liver Physiol 300: G241–G252, 2011.

    Derivative Assay:

    Article Title: Crosstalk between interleukin-6 and corticotropin-releasing factor modulate submucosal plexus activity and colonic secretion.
    Article Snippet: 0889-1591/$ see front matter 2013 Elsevier Inc. A http://dx.doi.org/10.1016/j.bbi.2013.01.078 Abbreviations: CRF, corticotropin releasing facto colo-rectal distension; GI, gastrointestinal; HPA, hyp IBS, irritable bowel syndrome; IL, interleukin; IL-6R short circuit current; JAK-STAT, janus tyrosine kinas activators of transcription; MAPK, mitogen activated separation; NS, non-separated; SD, standard deviatio mean; SMP, submucosal plexus; TER, trans-epithel necrosis factor.. ⇑ Corresponding author.. Address: Department o Gateway Building, University College Cork, Cork, Irela E-mail address: d.omalley@ucc.ie (D. O’Malley).

    Neutralization:

    Article Title: Colonic soluble mediators from the maternal separation model of irritable bowel syndrome activate submucosal neurons via an interleukin-6-dependent mechanism.
    Article Snippet: O’Malley D, Liston M, Hyland NP, Dinan TG, Cryan JF.. Colonic soluble mediators from the maternal separation model of irritable bowel syndrome activate submucosal neurons via an interleukin-6-dependent mechanism.. Am J Physiol Gastrointest Liver Physiol 300: G241–G252, 2011.

    Article Title: The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection.
    Article Snippet: Contents lists available at SciVerse ScienceDirect Experimental Neurology j ourna l homepage: www.e lsev ie r .com/ locate /yexnr The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection Xu-Hong Wei a,1, Xiao-Dong Na a,b,1, Guang-Jie Liao a, Qiu-Ying Chen a, Yu Cui a, Feng-Ying Chen c, Yong-Yong Li a, Ying Zang a,⁎, Xian-Guo Liu a,⁎ a Pain Research Center and Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University, PR China b Department of Pathophysiology, Zhongshan Medical School of Sun Yat-Sen University, PR China c School of Pharmaceutical Science of Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzhou 510080, PR China ⁎ Corresponding authors at: Department of Physiology Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzho E-mail addresses: yingzang@163.com (Y. Zang), liux 1 Both authors contribute equally to this work.

    Saline:

    Article Title: Inhibition of interleukin-6 attenuates erectile dysfunction in a rat model of nerve-sparing radical prostatectomy.
    Article Snippet: Introduction.. The precise mechanisms underlying erectile dysfunction (ED) occurring after cavernous nerve (CN)sparing surgery remain to be determined.. Aim.

    Article Title: The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection.
    Article Snippet: Contents lists available at SciVerse ScienceDirect Experimental Neurology j ourna l homepage: www.e lsev ie r .com/ locate /yexnr The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection Xu-Hong Wei a,1, Xiao-Dong Na a,b,1, Guang-Jie Liao a, Qiu-Ying Chen a, Yu Cui a, Feng-Ying Chen c, Yong-Yong Li a, Ying Zang a,⁎, Xian-Guo Liu a,⁎ a Pain Research Center and Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University, PR China b Department of Pathophysiology, Zhongshan Medical School of Sun Yat-Sen University, PR China c School of Pharmaceutical Science of Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzhou 510080, PR China ⁎ Corresponding authors at: Department of Physiology Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzho E-mail addresses: yingzang@163.com (Y. Zang), liux 1 Both authors contribute equally to this work.

    Control:

    Article Title: The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection.
    Article Snippet: Contents lists available at SciVerse ScienceDirect Experimental Neurology j ourna l homepage: www.e lsev ie r .com/ locate /yexnr The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection Xu-Hong Wei a,1, Xiao-Dong Na a,b,1, Guang-Jie Liao a, Qiu-Ying Chen a, Yu Cui a, Feng-Ying Chen c, Yong-Yong Li a, Ying Zang a,⁎, Xian-Guo Liu a,⁎ a Pain Research Center and Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University, PR China b Department of Pathophysiology, Zhongshan Medical School of Sun Yat-Sen University, PR China c School of Pharmaceutical Science of Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzhou 510080, PR China ⁎ Corresponding authors at: Department of Physiology Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzho E-mail addresses: yingzang@163.com (Y. Zang), liux 1 Both authors contribute equally to this work.

    Sterility:

    Article Title: The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection.
    Article Snippet: Contents lists available at SciVerse ScienceDirect Experimental Neurology j ourna l homepage: www.e lsev ie r .com/ locate /yexnr The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection Xu-Hong Wei a,1, Xiao-Dong Na a,b,1, Guang-Jie Liao a, Qiu-Ying Chen a, Yu Cui a, Feng-Ying Chen c, Yong-Yong Li a, Ying Zang a,⁎, Xian-Guo Liu a,⁎ a Pain Research Center and Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University, PR China b Department of Pathophysiology, Zhongshan Medical School of Sun Yat-Sen University, PR China c School of Pharmaceutical Science of Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzhou 510080, PR China ⁎ Corresponding authors at: Department of Physiology Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzho E-mail addresses: yingzang@163.com (Y. Zang), liux 1 Both authors contribute equally to this work.

    Concentration Assay:

    Article Title: The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection.
    Article Snippet: Contents lists available at SciVerse ScienceDirect Experimental Neurology j ourna l homepage: www.e lsev ie r .com/ locate /yexnr The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection Xu-Hong Wei a,1, Xiao-Dong Na a,b,1, Guang-Jie Liao a, Qiu-Ying Chen a, Yu Cui a, Feng-Ying Chen c, Yong-Yong Li a, Ying Zang a,⁎, Xian-Guo Liu a,⁎ a Pain Research Center and Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University, PR China b Department of Pathophysiology, Zhongshan Medical School of Sun Yat-Sen University, PR China c School of Pharmaceutical Science of Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzhou 510080, PR China ⁎ Corresponding authors at: Department of Physiology Sun Yat-Sen University, 74 Zhongshan Rd.. 2, Guangzho E-mail addresses: yingzang@163.com (Y. Zang), liux 1 Both authors contribute equally to this work.



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    Image Search Results


    Immunohistochemical analysis of pro-inflammatory cytokines IL-6 and TNF-α expression. Representative images of (A) IL-6 and (B) TNF-α staining (brown) in wound tissues from the Vaseline group, PRF group, Pigskin group, and PRF+pig skin group on Days 4, 7, 14, and 21. The Vaseline group shows sustained positive staining for both cytokines. In contrast, the Pigskin group and PRF+pig skin group exhibit markedly reduced staining intensity, particularly at days 14 and 21. Scale bar = 100 μm.

    Journal: Frontiers in Immunology

    Article Title: Synergistic effects of platelet-rich fibrin and CTLA4Ig gene-transfected porcine skin on accelerating wound healing in a rat model of deep second-degree burns: a mechanistic study

    doi: 10.3389/fimmu.2025.1756818

    Figure Lengend Snippet: Immunohistochemical analysis of pro-inflammatory cytokines IL-6 and TNF-α expression. Representative images of (A) IL-6 and (B) TNF-α staining (brown) in wound tissues from the Vaseline group, PRF group, Pigskin group, and PRF+pig skin group on Days 4, 7, 14, and 21. The Vaseline group shows sustained positive staining for both cytokines. In contrast, the Pigskin group and PRF+pig skin group exhibit markedly reduced staining intensity, particularly at days 14 and 21. Scale bar = 100 μm.

    Article Snippet: Briefly, after deparaffinization, rehydration, and antigen retrieval (citrate buffer, pH 6.0, for CD31 and VEGF; EDTA buffer, pH 9.0, for IL-6 and TNF-α), sections were incubated overnight at 4 °C with the respective primary antibodies: Rabbit anti-rat CD31 (1:500, Cat# 33075-1-AP, Proteintech), Rabbit anti-rat VEGF (1:500, Cat# 26157-1-AP, Proteintech), Rabbit anti-rat IL-6 (1:500, Cat# 21865-1-AP, Proteintech), and Rabbit anti-rat TNF-α (1:500, Cat# 17590-1-AP, Proteintech).

    Techniques: Immunohistochemical staining, Expressing, Staining

    Bar graph show serum levels of IL 6 & TNF-α markers between studied groups

    Journal: BMC Oral Health

    Article Title: Effect of bone marrow stem cell conditioned medium on amiodarone-induced submandibular salivary gland and kidney damage in male albino rats

    doi: 10.1186/s12903-025-07542-6

    Figure Lengend Snippet: Bar graph show serum levels of IL 6 & TNF-α markers between studied groups

    Article Snippet: ELISA assays were performed to measure serum levels of IL-6 and TNF- α using two monoclonal antibodies that were specific to different antigenic determinants on rat IL-6 (Quantikine® Colorimetric Sandwich ELISA Kits, Catalog Number R6000B) and rat TNF-α (CUSABIO®, Catalog Number.

    Techniques:

    TPM reverses neuroinflammation induced by ISIR. (A) Mechanism of TPM inhibiting the activation of the microglial cGAS‐STING pathway. (B) Schematic illustration of the HT22 cells and BV2 cells coincubation experimental process. (C) Immunofluorescence staining of dsDNA of BV2 cells in different treatment groups. (D) Immunohistochemical staining of STING in the cerebral infarction area of each treatment group. Scale bar: 20 µm. (E) Quantitative analysis of STING immunohistochemical staining. (F) WB of inflammation‐related proteins in cerebral infarct area, including cGAS, STING, IRF3, P‐IRF3, P65, and P‐P65. (G–K) Determination of the levels of pro‐inflammatory factors (IL‐1β, IL‐6, and TNF‐α) and anti‐inflammatory factors (IL‐4 and IL‐10) in the cerebral infarction area of each treatment group. (L–O) Quantitative analysis of inflammation‐related proteins in BV2 cells, including cGAS (l), STING (m), P‐IRF3/ IRF3 (N), P‐P65/ P65 (O). Data were expressed as mean ± SE. ( n = 3, ns, p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001).

    Journal: Exploration

    Article Title: Specifically Breaking Through the Injured Blood‐Brain Barrier With Tannic Acid‐Based Nanomedicine for Ischemic Stroke Ischemia Reperfusion Treatment

    doi: 10.1002/EXP.20240388

    Figure Lengend Snippet: TPM reverses neuroinflammation induced by ISIR. (A) Mechanism of TPM inhibiting the activation of the microglial cGAS‐STING pathway. (B) Schematic illustration of the HT22 cells and BV2 cells coincubation experimental process. (C) Immunofluorescence staining of dsDNA of BV2 cells in different treatment groups. (D) Immunohistochemical staining of STING in the cerebral infarction area of each treatment group. Scale bar: 20 µm. (E) Quantitative analysis of STING immunohistochemical staining. (F) WB of inflammation‐related proteins in cerebral infarct area, including cGAS, STING, IRF3, P‐IRF3, P65, and P‐P65. (G–K) Determination of the levels of pro‐inflammatory factors (IL‐1β, IL‐6, and TNF‐α) and anti‐inflammatory factors (IL‐4 and IL‐10) in the cerebral infarction area of each treatment group. (L–O) Quantitative analysis of inflammation‐related proteins in BV2 cells, including cGAS (l), STING (m), P‐IRF3/ IRF3 (N), P‐P65/ P65 (O). Data were expressed as mean ± SE. ( n = 3, ns, p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001).

    Article Snippet: Rat IL‐6 (Interleukin 6) ELISA kit , Elabscience , E‐EL‐R0015.

    Techniques: Activation Assay, Immunofluorescence, Staining, Immunohistochemical staining